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Dioclea Altissima Seed Lectin (DAL) Prevents Anxiety-like Behavioral Responses in Adult Zebrafish (Danio Rerio): Involvement of GABAergic and 5-HT Systems

Author(s):

João Ronielly Campêlo Araújo, Adriana Rolim Campos, Maria Kueirislene Amâncio Ferreira, Sacha Aubrey Alves Rodrigues Santos, Marina de Barros Mamede Vidal Damasceno, Francisco Ernani Alves Magalhães, Renato de Azevedo Moreira and Ana Cristina de Oliveira Monteiro-Moreira*   Pages 1 - 9 ( 9 )

Abstract:


Background: Plant lectins have shown promising neuropharmacological activities in animal models.

Objective: This study evaluated the effect of Dioclea altissima seed lectin (DAL) on adult zebrafish behavior.

Method: Zebrafish (n=6/group) were treated (i.p.; 20 μL) with DAL (0.025; 0.05 or 0.1 mg/mL), vehicle or diazepam (DZP) and submitted to several tests (open field, light/dark preference or novel tank). Flumazenil, pizotifen or granisetron were administered 15 min before DAL (0.05 mg/mL), and the animals were evaluated on light/dark preference test. It was also verified whether the DAL effect depended on its structural integrity and ability to interact with carbohydrates.

Results: DAL decreased the locomotor activity of adult zebrafish (0.025; 0.05 or 0.1 mg/mL), increased the time spent in the upper region of the aquarium (0.025 mg/mL), and decreased the latency time of adult zebrafish to enter the upper region on the novel tank test. DAL (0.05 mg/mL) also increased their permanence in the light zone of the light/dark preference test. The effect of DAL was dependent on carbohydrate interaction and protein structure integrity and was prevented by pizotifen, granizetron and flumazenil.

Conclusion: DAL was found to have an anxiolytic-like effect mediated by the 5-HT and GABAergic receptors.

Keywords:

Dioclea altissima, lectin, neurobehavioral, anxiolytic-like, 5-HT receptors, GABAergic receptors.

Affiliation:

Rede Nordeste de Biotecnologia (Renorbio), State University of Ceará (UECE), Fortaleza, Ceará, Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Rede Nordeste de Biotecnologia (Renorbio), State University of Ceará (UECE), Fortaleza, Ceará, Laboratory of Natural Products Chemistry, Synthesis and Biocatalysis of Organic Compounds (LBPNSB), State University of Vale do Acaraú, Betânia Campus, Sobral, Ceará, Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará



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