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CSF Levels of the Endocannabinoid Anandamide are Reduced in Patients with Untreated Narcolepsy Type 1: A Pilot Study

[ Vol. 19 , Issue. 2 ]

Author(s):

Andrea Romigi*, Monica Bari, Claudio Liguori, Francesca Izzi, Cinzia Rapino, Marzia Nuccetelli, Natalia Battista, Sergio Bernardini, Diego Centonze, Nicola Biagio Mercuri, Fabio Placidi and Mauro Maccarrone   Pages 142 - 147 ( 6 )

Abstract:


Background: Endocannabinoids (ECs) modulate both excitatory and inhibitory components in the CNS. There is a growing body of evidence that shows ECs influence both hypothalamic orexinergic and histaminergic neurons involved in narcolepsy physiopathology. Therefore, ECs may influence sleep and sleep-wake cycle.

Objective: To evaluate EC levels in the CSF of untreated narcoleptic patients to test whether ECs are dysregulated in Narcolepsy Type 1 (NT1) and Type 2 (NT2).

Methods: We compared CSF Anandamide (AEA), 2-Arachidonoylglycerol (2-AG) and orexin in narcoleptic drug-naïve patients and in a sample of healthy subjects.

Results: We compared NT1 (n=6), NT2 (n=6), and healthy controls (n=6). We found significantly reduced AEA levels in NT1 patients compared to both NT2 and controls. No differences were found between AEA levels in NT2 versus controls and between 2-AG levels in all groups, although a trend toward a decrease in NT1 was evident. Finally, the CSF AEA level was related to CSF orexin levels in all subjects.

Conclusion: We demonstrated that the EC system is dysregulated in NT1.

Keywords:

Endocannabinoid, narcolepsy type 1, narcolepsy type 2, anandamide, 2-arachidonoylglycerol, cerebrospinal fluid.

Affiliation:

IRCCS Neuromed, Sleep Medicine Center, Via Atinense 18, University of Rome Tor Vergata, Department of Experimental Medicine, Via Montpellier 1, University of Rome Tor Vergata, System Medicine, Via Montpellier 1, University of Rome Tor Vergata, System Medicine, Via Montpellier 1, University of Teramo, Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Rome Tor Vergata, Department of Experimental Medicine, Viale Oxford 81, University of Teramo, Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Rome Tor Vergata, Department of Experimental Medicine, IRCCS Neuromed, Neurology Department, Via Atinense 18, University of Rome Tor Vergata, System Medicine, Via Montpellier 1, University of Rome Tor Vergata, System Medicine, Via Montpellier 1, Campus Bio-Medico University of Rome, Department of Experimental Medicine



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