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Exosomal miR-214-5p Released from Glioblastoma Cells Modulates Inflammatory Response of Microglia after Lipopolysaccharide Stimulation through Targeting CXCR5

[ Vol. 18 , Issue. 1 ]

Author(s):

Jian-kai Yang*, Hong-jiang Liu, Yuanyu Wang, Chen Li, Ji-peng Yang, Liang Yang, Xue-jiao Qi, Yin-long Zhao, Xue-fang Shi, Jing-chen Li, Guo-zhu Sun and Bao-hua Jiao   Pages 78 - 87 ( 10 )

Abstract:


Background and Objective: Exosomes communicate inter-cellularly and miRNAs play critical roles in this scenario. MiR-214-5p was implicated in multiple tumors with diverse functions uncovered. However, whether miR-214-5p is mechanistically involved in glioblastoma, especially via exosomal pathway, is still elusive. Here we sought to comprehensively address the critical role of exosomal miR-214-5p in glioblastoma (GBM) microenvironment.

Methods: The relative expression of miR-214-5p was determined by real-time PCR. Cell viability and migration were measured by MTT and transwell chamber assays, respectively. The secretory cytokines were measured with ELISA kits. The regulatory effect of miR-214-5p on CXCR5 expression was interrogated by luciferase reporter assay. Protein level was analyzed by Western blot.

Results: We demonstrated that miR-214-5p was aberrantly overexpressed in GBM and associated with poorer clinical prognosis. High level of miR-214-5p significantly contributed to cell proliferation and migration. GBM-derived exosomal miR-214-5p promoted inflammatory response in primary microglia upon lipopolysaccharide challenge. We further identified CXCR5 as the direct target of miR-214- 5p in this setting.

Conclusion: Overexpression of miR-214-5p in GBM modulated the inflammatory response in microglia via exosomal transfer.

Keywords:

miR-214-5p, glioblastoma, microglia, CXCR5, cytokine, brain tumor.

Affiliation:

Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000

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