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Notoginsenoside R1 Promotes the Growth of Neonatal Rat Cortical Neurons via the Wnt/β-catenin Signaling Pathway

[ Vol. 17 , Issue. 7 ]

Author(s):

Xing-Tong Li, Wei Ma, Xian-Bin Wang, Zhang Liang, Jin-Wei Yang, Yuan He, Yun-Fei Dai, Tong Zhang, Jia-Wei Wang, Kuang-Pin Liu, Xiang-Peng Wang, Xing-Kui Zhang, Chun-Yan Li, Jun-Jun Li, Hong-Yan Tang, Ping Su*, Jian-Hui Guo* and Li-Yan Li*   Pages 547 - 556 ( 10 )

Abstract:


Background & Objective: Notoginsenoside R1 (NGR1) is one of the main effective components of Panax notoginseng. Primary cortical neurons were harvested from neonatal rats and cultured to analyze the role of NGR1 in neuronal growth and the effects of NGR1 on the Wnt/β-catenin signaling pathway. Following treatment with NGR1, immunocytochemistry was used to detect expression of Tuj1 and MAP2, and RT-qPCR was used to measure mRNA levels of key factors in the Wnt signaling pathway. Results showed that NGR1 promotes growth of cultured neurons and significantly up-regulates mRNA levels of β-catenin, Dishevelled, and Frizzled. To further confirm whether NGR1 promoted cortical neuron growth via the Wnt/β-catenin signaling pathway, we knocked down β- catenin mRNA by siRNA interference; following NGR1 treatment of β-catenin-knockdown neurons, β-catenin mRNA levels increased significantly.

Conclusion: In conclusion, these results demonstrate that NGR1 promotes growth of cultured cortical neurons from the neonatal rat, possibly via the Wnt/β-catenin signaling pathway.

Keywords:

Wnt/β-catenin signaling pathway, β-catenin, NGR1, growth, cortical neuron, RNA interference.

Affiliation:

Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Department of gastroenterology, First People's Hospital of Yunnan Province, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, 2Second Department of General Surgery, First People's Hospital of Yunnan Province, Yunnan Kunming, 2Second Department of General Surgery, First People's Hospital of Yunnan Province, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming, Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Yunnan Kunming, Department of Neurosurgery, First People's Hospital of Kunming City, Yunnan Kunming, Second Department of General Surgery, First People's Hospital of Yunnan Province, Yunnan Kunming, Institute of Neuroscience, Kunming Medical University, Yunnan Kunming



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