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Upregulation of Connexin-43 is Critical for Irradiation-induced Neuroinflammation

[ Vol. 17 , Issue. 7 ]

Author(s):

Chen Wei, Wusong Tong, Yijun Guo, Bin He, Lei Chen, Wenjin Yang, Chenxing Wu, Dabin Ren, Ping Zheng* and Jiugeng Feng*   Pages 539 - 546 ( 8 )

Abstract:


Background: Radiation therapy is widely used for the treatment of pituitary adenomas. Unfortunately, it might raise the risk of ischemic stroke, with neuroinflammation being a major pathological process. Astrocytes are the most abundant cell type in the central nervous system and have been reported for playing important roles in ischemic stroke.

Objective: Here we studied how γ-radiation would introduce astrocytes into a detrimental state for neuroinflammation and provide new theory evidence and target for the clinical management of inflammation- related neural damage after radiation-induced ischemic stroke.

Method: HA-1800 cells were treated with γ-radiation and then the protein and mRNA levels of Connexin (Cx)-43 were evaluated by western and q-PCR. The culture supernatant was collected and the concentrations of the inflammatory factors were determined by ELISA. MiRNA complementary to Cx-43 was designed through the online tools.

Results: Cx-43 is upregulated in the treatment of γ-radiation in astrocytes and γ-radiation introduced the detrimental function of astrocytes: cell viability was reduced while the apoptotic cells were increased. Inflammatory factors like tumor necrosis factor alpha, interferon gamma, interleukin-6, interleukin 1-beta were dramatically up-regulated by the irradiation. MiR-374a rescued irradiation induced Cx-43 up-regulation of astrocytes and eliminated detrimental function triggered by γ-radiation.

Conclusion: Cx-43 expression level may play an important role in the inflammation-related neural damage after irradiation-induced ischemic stroke.

Keywords:

Ischemia, Stroke, Cx-43, Astrocytes, MiR-374a.

Affiliation:

The People’s Hospital of Pudong New Area, 490 South Chuanhuan Road, Chuansha New Town, Shanghai 201299, The People’s Hospital of Pudong New Area, 490 South Chuanhuan Road, Chuansha New Town, Shanghai 201299, The People’s Hospital of Pudong New Area, 490 South Chuanhuan Road, Chuansha New Town, Shanghai 201299, The People’s Hospital of Pudong New Area, 490 South Chuanhuan Road, Chuansha New Town, Shanghai 201299, The People’s Hospital of Pudong New Area, 490 South Chuanhuan Road, Chuansha New Town, Shanghai 201299, The People’s Hospital of Pudong New Area, 490 South Chuanhuan Road, Chuansha New Town, Shanghai 201299, The People’s Hospital of Pudong New Area, 490 South Chuanhuan Road, Chuansha New Town, Shanghai 201299, The People’s Hospital of Pudong New Area, 490 South Chuanhuan Road, Chuansha New Town, Shanghai 201299, Shanghai University of Medicine & Health Sciences, 279 ZhouZhu Road, Pudong New Area, 200120, Shanghai, Department of Neurosurgery, The First Affiliated Hospital of Nanchang University, Yong Wai Zheng Street 17, Nanchang 330006



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