Kin Yen Chia, Khuen Yen Ng, Rhun Yian Koh and Soi Moi Chye* Pages 1 - 9 ( 9 )
Background & Objective: Protein misfolding and aggregation have been considered the common pathological hallmarks for a number of neurodegenerative diseases, including Alzheimer’s disease (AD), Parkinson’s disease (PD) and Huntington’s disease (HD). These abnormal proteins aggregation damages mitochondria and induces oxidative stress, resulting in neuronal cell death. Prolongeddd neuronal damage activates microglia and astrocytes, development of inflammation reaction and further promotes neurodegeneration. Thus, elimination of abnormal proteins aggregation without eliciting any adverse effects are the main treatment strategies. To overcome this, recent studies have deployed single-chain fragment variable antibodies (scFv) to target the pathological protein aggregates, such as amyloid-beta (Aβ) peptides, α-synuclein (α-syn) and Huntingtin (Htt). To date scFv has been effective at inhibiting abnormal protein aggregates formation in both in vitro and in vivo model system of AD, PD and HD.
Conclusion: Currently active research is still ongoing to improve the scFv gene delivery technology, to further enhance brain penetration, intracellular stability, solubility and efficacy of scFv intrabody.
single-chain Fv antibodies, neurodegenerative disease, oxidative stress, inflammation reaction
International Medical University - Applied Biomedical Science and Biotechnology Kuala Lumpur, Monash University Malaysia - School of Pharmacy Kuala Lumpur, International Medical University - Applied Biomedical Science and Biotechnology Kuala Lumpur, International Medical University - Applied Biomedical Science and Biotechnology Kuala Lumpur