Article Details


Curcumin Suppresses Tumor Growth and Angiogenesis in Human Glioma Cells Through Modulation of Vascular Endothelial Growth Factor/ Angiopoietin-2/Thrombospondin-1 Signaling

[ Vol. 16 , Issue. 3 ]

Author(s):

Zhiqiang Zhang, Cong Li, Qijia Tan, Caijun Xie, Yanying Yang, Wengang Zhan, Fu Han, Hari Shanker Sharma and Aruna Sharma   Pages 346 - 350 ( 5 )

Abstract:


Purpose: To investigate the effect of curcumin on tumor growth and angiogenesis of human gliomas and identify the underlying molecular mechanisms.

Methods: A mouse xenograft glioma model was established by subcutaneously inoculating tumor cell aggregates derived from the U87 cell line. Mice were treated with 0.01ml/g body weight of curcumin or saline. Tumor volume was measured. Microvessel density was assessed by CD34 immunostaining, and angiogenesis by immunohistochemical staining of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2) and thrombospondin 1 (TSP-1).

Results: At 28 days after treatment, tumor weights in the curcumin-treated group were much smaller than in the control group (0.23±0.11g vs 0.44±0.15g,p<0.05), resulting in a 45.8% inhibition of tumor growth. Curcumin also markedly inhibited microvessel density. Expression of VEGF and Ang-2 was inhibited by curcumin, whereas TSP-1 expression was up-regulated.

Conclusion: This study shows that curcumin inhibits tumor growth by inhibiting VEGF/Ang-2/TSP-1- mediated angiogenesis in a xenograft glioma mouse model.

Keywords:

Curcumin, glioma, angiogenesis, vascular endothelial growth factor.Curcumin, glioma, angiogenesis, vascular endothelial growth factor.

Affiliation:

Director of Research, CNS Injury & Repair (IECNSIR), Uppsala University Hospital, Uppsala University, Frödingsgatan 12, Bldg. 1228, SE-7541 Uppsala, Department of Neurosurgery, Chinese Medicine Hospital of Guangdong Province, The Second Affiliated Hospital, University of Chinese Medicine in Guangzhou, Guangzhou, Department of Neurosurgery, Chinese Medicine Hospital of Guangdong Province, The Second Affiliated Hospital, University of Chinese Medicine in Guangzhou, Guangzhou, Department of Neurosurgery, Chinese Medicine Hospital of Guangdong Province, The Second Affiliated Hospital, University of Chinese Medicine in Guangzhou, Guangzhou, Department of Neurosurgery, Chinese Medicine Hospital of Guangdong Province, The Second Affiliated Hospital, University of Chinese Medicine in Guangzhou, Guangzhou, Department of Neurosurgery, Chinese Medicine Hospital of Guangdong Province, The Second Affiliated Hospital, University of Chinese Medicine in Guangzhou, Guangzhou, Department of Neurosurgery, Chinese Medicine Hospital of Guangdong Province, The Second Affiliated Hospital, University of Chinese Medicine in Guangzhou, Guangzhou, Director of Research, CNS Injury & Repair (IECNSIR), Uppsala University Hospital, Uppsala University, Frödingsgatan 12, Bldg. 1228, SE-7541 Uppsala, International Experimental Central Nervous System Injury & Repair (IECNSIR), Laboratory of Cerebrovascular Research, Department of Surgical Sciences Anesthesiology and Intensive Care Medicine, University Hospital, Uppsala University, SE-75421 Uppsala

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